JAK3 Knockout cell line (Jurkat)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

JAK3 Knockout cell line (Jurkat) Order

Catalog#

YKO-H763

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

JAK3 Knockout cell line (Jurkat, Clone E6-1)

Morphology

Lymphocyte-like,suspension

Passage ratio

1:3-1:5

Species

Human

Gene Name

JAK3

Gene ID

3718

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Target region

Knockout region: Exon 2 (based on transcript JAK3-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Jurkat, Clone E6-1			STR Info (Cell bank) Cell Line:Jurkat, Clone E6-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	9	9		9	9
D13S317	8	12		8	12
D7S820	8	12		8	12
D16S539	11	11		11	11
VWA	18	18		18	18
TH01	6	9.3		6	9.3
AMEL	X	Y		X	Y
TPOX	8	10		8	10
CSF1PO	11	12		11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the JAK3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Jurkat, Clone E6-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, JAK3 gene knockout JAK3 Knockout cell line (Jurkat, Clone E6-1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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